:: What is Nattokinase ? ::
Nattokinase(NK) is a potent fibrinolytic enzyme (anti-blood clotting) found in a traditional Japanese food called 『NATTO』. A fermentation process produces Natto by inoculating Bacillus subtilis natto, a GRAS beneficial bacteria, to cooked soybeans. It is only the natto preparation that contains the specific nattokinase-a protease enzyme. |
:: The Discovery of Nattokinase ::
Dr. Hiroyuki Sumi discovered nattokinase in 1980 while working as a visiting scientist and majoring in physiological chemistry at Chicago University Medical School . Dr. Sumi found what he was looking for when Natto extract was dropped onto artificial thrombus(fibrin) in a Petri dish and allowed it to stand at 37°C. The thrombus around the natto dissolved gradually, and completely dissolved within 18 hours. Dr. Sumi named the newly discovered enzyme 『Nattokinase』, which means 『enzyme in natto』. Sumi commented that nattokinase showed 『a potency matched by no other enzyme』.
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:: The Fibrinolytic Activity Assay by Vedan's Nattokinase
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:: The Manufacturing Process of Vedan's Nattokinase ::
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:: Product Quality Control ::
 Non GMO Soybean Protein
Careful selection of the material - soybean protein for fermentation. It is certified by “Food Industry Research and Development Institute, (FIRDI)?
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| Test Number |
94GM009 |
| Sample Name |
Soybean protein |
| Screening test |
35S promoter gene |
Negative |
| NOS terminator gene |
Negative |
| Lectin gene |
Positive |
| Confirmation test |
EPSPS gene |
Negative |
| The detection limit of test for EPSPS gene and the Lectin gene is 0.1%. The detection limit of test for the Tnos and P35S gene is 0.01%. |
| Summary |
The sample does not contain GMO soybean. |
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The Safety of Bacteria
The Bacillus subtilis natto that we use to produce high quality, highly productive Nattokinase is certified by FIRDI, report file number 93ID053 (see fig. 1). In food industry, Bacillus subtilis natto as well as lactic acid bacteria are both identified as non-toxic, non-pathogenic bacteria that conforms to food safely standard. |
Stable Activity
Nattokinase is highly active and stable. The Japanese authority, JFRL (Japanese Food Research Laboratories) has already testified and confirmed its benign nature.
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| Test Number |
Sample |
Analysis Item |
Result |
Analysis Method |
| 204081399-002 |
Type I |
Nattkinase activity |
1.7×10 FU/g |
Fibrin-digestion method * |
| 204111561-001 |
Type II |
Nattkinase activity |
4.0×10FU/g |
Fibrin-digestion method * |
| *:One FU is defined as the activity that will increase the absorbance at 275 nm by 0.01 per minute at the beginning of the reaction time under the following conditions : pH 8.5, temperature 37°C , substrate : fibrinogen-thrombin. |
Toxicity Study 
The acute oral toxicity study in rats done by DCB, test serial number is DV-TR-AC00197. In conclusion, single oral administration of “nattokinase powder?exert no toxic effect on Sprague-Dawley (SD) rats up to the does level of 5,000 mg/kg during the 14-day observation period, LD > 5,000 mg/kg.
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:: Product Specification ::
| Item |
Type I |
Type II |
| Appearance |
Brownish powder |
Pale-yellowish to brownish powder |
| Odor |
Fermented natto odor |
Slight fermented natto odor |
| Loss on Drying |
<10% |
<10% |
| Nattokinase Activity |
>12,000FU/g |
>20,000 FU/g |
| Isoflavone-Genistein |
135 10 /g |
Not Detected |
| Isoflavone-Daidzein |
24010/g |
Not Detected |
| Isoflavone-Glycitein |
5010/g |
Not Detected |
| Vitamin K2 |
<500ppm |
<1ppm |
| E .Coli & Coliform |
Negative |
Negative |
| Salmonella |
Negative |
Negative |
| Heavy metals |
Total as Pb:<20ppm |
Total as Pb:<20 ppm |
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